A Xavier Turner sacrifice fly in the third pushed the Freedom lead to 3-0.
Austin Wobrock further padded the Freedom lead with a two-run homer in the fourth.
The Otters finally got on the board in the seventh when Travis Harrison swatted a solo home run, his fourth of the year, to cut the deficit to 5-1.
In the ninth, the Otters tried to mount a comeback as Jeff Gardner plated Toby Thomas with an RBI single, but the rally would fall short as the Otters dropped the series finale 5-2.
Randy Wynne takes his third loss of the season for the Otters. Wynne threw an eight-inning complete game, allowing five runs, on four hits, while striking out four and not walking a hitter.
Freedom starter Christian DeLeon picks up the win after throwing eight innings of one-run baseball. DeLeon limited the Otters to just three hits and struck out three hitters.
Evansville now returns home to begin a three-game series with the Southern Illinois Miners at 6:35 p.m. tomorrow at Bosse Field.